Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Human T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Takara Bio PrimeSTAR® Max DNA Polymerase, 100 Rxns
A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition. 100 Rxns
Bio Rad Laboratories POLYPRP COLMN 2ML 9CM LNG 50PK
Poly-Prep Columns, 50pk, Height 9cm, Hold up to 2mL of chromatography media and 10mL or eluent or sample in an integral reservoir, Includes end caps and tip closures
STEMCELL Technologies EasySep™ RBC Depletion Reagent - 10mL, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
IBA LifeSciences Strep-TactinXT 4Flow resin as 50% suspension; 20 ml
Strep-TactinXT 4Flow is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Aniara Diagnostica LLC ANTI - XA
This BIOPHEN™ ANTI-Xa (2 Stages heparin Assay) kit is a two-stage chromogenic assay for measuring the activity of heparins (UFH or LMWH), in manual or automatic method. This method is proposed only to test heparin in human citrated plasma, or in purified solution. This kit is for research use only and must not be used from patient diagnosis or treatment. NOTE: This product replaces Ref #221010 and requires separate purchase of specific buffers depending on the analyte being tested and compendia reference desired: Specific buffer needed for UFH Assay, USP or EP: AR030K Specific buffers needed for LMWH Assay, USP: AR005L, AR029K, AR028K Specific buffers for LMWH Assay, EP: AR005L, AR029K
EPICYPHER INC CUTANA™ Concanavalin A Conjugated Paramagnetic Beads | 50uL - 50rxns
This product contains Concanavalin A-conjugated to paramagnetic microspheres. Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) that binds specifically to mannosyl- and glucosyl-containing extracellular glycoproteins. The ConA magnetic beads are therefore useful to immobilize cells or nuclei presenting these glycans in their extracellular matrices.
STEMCELL Technologies EasySep™ Human CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human CD8+ T Cell Enrichment Kit (Catalog #19053) for even faster cell isolations.
IBA LifeSciences Strep-TactinXT 4Flow high capacity resin as 50% suspension; 50 ml
Strep-TactinXT 4Flow high capacity is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity, the resin is recommend for all protein classes, especially for low abundant proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
STEMCELL Technologies EasySep™ Direct Human Neutrophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Neutrophil Isolation Kit isolates functional, highly purified neutrophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.This product replaces the EasySep™ Human Neutrophil Isolation Kit (Catalog #19257) for even faster cell isolations.
IBA LifeSciences Strep-TactinXT 4Flow high capacity cartridge; 5 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.
Sigma Aldrich Fine Chemicals Biosciences Nuclei Isolation Kit: Nuclei EZ Prep
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types. Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
Sigma Aldrich Fine Chemicals Biosciences IGEPAL CA-630, 9002-93-1, MFCD00132505, 50 mL
Linear Formula: (C2H4O)nC14H22O, for molecular biology, Synonym: Octylphenoxy poly(ethyleneoxy)ethanol, branched. IGEPAL CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.
Dojindo Molecular Technologies Inc Dojindo ExoSparkler Exosome Protein Labeling Kit, Red, 1 to 10 μg Protein per Sample, 5 Samples
This kit allows for exosome protein fluorescent staining and enables observation of exosome dynamics without extracellular aggregation, covering steps from fluorescence labeling to purification. It can be used for staining purified exosomal membrane or protein, allowing imaging of labeled exosomes taken up by cells.
- Enables observation of exosome dynamics without extracellular aggregation.
- Includes filtration tubes for removal of unreacted dyes.
- Optimized protocol for exosome labeling.
- Recommended protein amount per sample: 1 to 10 μg.
- Recommended particle count per sample: 10 to 100 x 108 particles.
- Detection conditions (red): Ex 561 nm/Em 570 to 640 nm.
- Recommended purification methods include ultracentrifugation, immunoprecipitation, and magnetic beads.
- Store at 0°C to 5°C, protected from moisture.