Protein Purification Reagents and Kits
Aniara Diagnostica LLC ANTI - XA
This BIOPHEN™ ANTI-Xa (2 Stages heparin Assay) kit is a two-stage chromogenic assay for measuring the activity of heparins (UFH or LMWH), in manual or automatic method. This method is proposed only to test heparin in human citrated plasma, or in purified solution. This kit is for research use only and must not be used from patient diagnosis or treatment. NOTE: This product replaces Ref #221010 and requires separate purchase of specific buffers depending on the analyte being tested and compendia reference desired: Specific buffer needed for UFH Assay, USP or EP: AR030K Specific buffers needed for LMWH Assay, USP: AR005L, AR029K, AR028K Specific buffers for LMWH Assay, EP: AR005L, AR029K
ProteinSimple Maurice cIEF pI Marker - 8.40, Lyophilized, 210 uL
Maurice C. reagents and consumables are designed for use on with Maurice and Maurice C. systems, and must be used in conjunction with Compass for iCE version 1.0 software.
Macherey-Nagel Protino Ni-NTA Agarose, 25 mL
Protino Ni-NTA Agarose (25 mL) Protino Ni-NTA suspension for the purification of His-tag proteins pack of 25 mL settled beaded agarose
Sigma Aldrich Fine Chemicals Biosciences IGEPAL CA-630, 9002-93-1, MFCD00132505, 50 mL
Linear Formula: (C2H4O)nC14H22O, for molecular biology, Synonym: Octylphenoxy poly(ethyleneoxy)ethanol, branched. IGEPAL CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.
Pfanstiehl Inc Maltose Monohydrate. High Purity, Low Endotoxin, Low Metals, USP-NF, JP, ChP (M-167) -100G GMP, Multi-compendial CAS: 6363-53-7
Pfanstiehl Maltose Monohydrate High Purity, Low Endotoxin, Low Metals is a multi-compendial carbohydrate utilized in bioprocessing applications and as an infusion stabilizer. Pfanstiehl Maltose has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Maltose meets parenteral (injectable) excipient grade standards, making it ideal as a bioprocessing feed, or as a stabilizing agent for pharmaceutical infusions. Pfanstiehl Maltose is available as USP-NF (National Formulary) JP (Japanese Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
Teknova Studier ZYM-5052 Medium Kit. 1L Kit
Studier ZYM-5052 Medium Kit, To complete media aseptically add 60mL of 3S2600 to 3S2200, Media is stable 2 months after salts are added
STEMCELL Technologies EasySep™ Direct Human PBMC Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human PBMC Isolation Kit is designed to isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis products by negative selection. Red blood cells (RBCs), platelets, and unwanted cells are targeted for removal with antibody complexes and magnetic particles and separated using an EasySep™ magnet. Untouched PBMCs are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
STEMCELL Technologies EasySep™ RBC Depletion Reagent - 10mL, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
Invent Biotechnologies Inc Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues, Catalog Number LY-034, 20 preps, 20 tests, Protocol Time less than 90 min
Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues, Catalog Number LY-034, 20 preps, 20 tests, Protocol Time less than 90 min
IBA LifeSciences Strep-TactinXT 4Flow high capacity resin as 50% suspension; 50 ml
Strep-TactinXT 4Flow high capacity is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity, the resin is recommend for all protein classes, especially for low abundant proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
ARCTICZYMES Endonucleases Non-Specific, HL-SAN concentration 25U /uL
Endonucleases Non-Specific, HL-SAN concentration 25U /uL
Izon Science Usa Ltd qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
IBA LifeSciences Strep-TactinXT 4Flow high capacity cartridge; 5 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.
Neuromics, Inc BioLock Biotin Blocking Solution, 50 ml
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.Activity: >70U/mlAdd at least 1U of BioLock solution per µg of biotin.